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Open Access Article

Journal of Cell and Molecular Biology Research. 2021; 1: (1) ; 4-11 ; DOI: 10.12208/j.ijcmbr.20210002.

Bioinformatic Analysis of Trans-cinnamate 4-monooxygenase Gene C4H in Bletilla striata
白及反式-肉桂酸4-单加氧酶基因C4H的序列分析

作者: 赵永彬, 黄恻隐, 徐程, 钱露, 李清清, 何毅怀, 徐德林*,

遵义医科大学珠海校区 广东珠海

遵义医科大学细胞生物学教研室 贵州遵义

遵义医科大学附属医院 贵州遵义

*通讯作者: 徐德林,单位:遵义医科大学细胞生物学教研室 贵州遵义;

发布时间: 2021-06-30 总浏览量: 148

摘要

反式-肉桂酸4-单加氧酶是植物中苯丙素和木质素合成的关键酶之一,研究C4H基因的序列信息对揭示其蛋白结构及功能具有重要的指导意义。从白及转录组数据库中分离得到BsC4H基因,运用生物信息学软件对该基因进行序列分析。分析得到的结果显示:该基因长度为1993 bp,编码的蛋白质长度为505 aa、分子量为58.01858 kD、理论等电点为9.20,该基因的二级结构主要由α螺旋结构和无规则卷曲结构组成,三级结构预测为单体结构。氨基酸序列与铁皮石斛C4H基因相似性最高,达95%,结构域分析发现该蛋白具有高度结构保守域,但并不包含有跨膜结构域和信号肽,亚细胞定位分析发现该酶被定位于内质网中。该研究结果为研究白及BsC4H基因提供了基础数据,也为研究其它物种C4H基因提供基础资料。

关键词: 白及;反式-肉桂酸4-单加氧酶;序列分析;结构预测

Abstract

Trans-cinnamic acid 4-monooxygenase is one of the key enzymes in the synthesis of phenylpropanoid and Lignin in plants. The C4H gene of Bletilla striata (BsC4H) was isolated from our former constructed transcriptome database and then analyzed by a series of bioinformatics software. The results showed that the length of the gene was 1993 bp, which encoding a protein with 505 aa in length which have molecular weight of 58.01858 kd and theoretical isoelectric point of 9.20. The predicted secondary structure of this protein was composed of helix structure and irregular coil structure, the tertiary structure was predicted to be monomer structure. BsC4H sequences of amino acid showed 95% similarity to C4H gene derived from Dendrobium officinale. The domain detection showed that BsC4H protein had a highly conserved domain, but no transmembrane domain and signal peptide. While the detection of subcellular localization found BsC4H was mainly functioned in the endoplasmic reticulum. The results of this study provided basic data for the subsequent study of BsC4H and developed inspirment for of C4H studies of other species.

Key words: Bletilla striata; Trans-cinnamate 4-monooxygenase; sequence analysis; structure prediction

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引用本文

赵永彬, 黄恻隐, 徐程, 钱露, 李清清, 何毅怀, 徐德林, 白及反式-肉桂酸4-单加氧酶基因C4H的序列分析[J]. 细胞与分子生物学研究, 2021; 1: (1) : 4-11.