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当前位置：细胞与分子生物学研究 > 2021年 1卷 (1)期

Open Access Article

Journal of Cell and Molecular Biology Research. 2021; 1: (1) ; 26-32 ; DOI: 10.12208/j.ijcmbr.20210006.

Bioinformatics Analysis of PANK in Bletilla striata
白及泛酸激酶基因的生物信息学分析

作者： 李清清, 黄恻隐, 范娠源, 刘海 *, 汪牧非, 徐德林

遵义医科大学细胞生物学教研室贵州遵义

贵州省现代中药材研究所贵州贵阳

*通讯作者：刘海,单位：贵州省现代中药材研究所贵州贵阳；

国家自然科学基金项目贵州省科学技术基金项目遵义市科技局遵义医科大学科学技术联合资金招标专项

发布时间: 2021-08-23 总浏览量: 1450

PDF 全文下载引用本文收录截图（CNKI-Scholar）

摘要

基于白及的转录组测序数据，采用生物信息学方法对白及PanK进行基因鉴定和序列分析。分析了白及PANK蛋白的等电点、亲疏水性以及二级结构、三级结构和结构域，进行亚细胞定位并预测其磷酸化位点、信号肽和跨膜区，最后，构建进化树分析。结果表明：白及PanK基因包含2697 bp的开放阅读框，共编码898个氨基酸，该蛋白相对分子质量为99405.55 Da，是等电点（pI）为5.79的亲水蛋白质。该蛋白有11个苏氨酸磷酸化位点，具有泛酸激酶（PLN02902家族蛋白）保守结构域，含10个功能motif；无信号肽及跨膜区，亚细胞定位发现该蛋白定位于细胞质中；进化树分析表明白及与铁皮石斛的PanK基因亲缘关系最近。结论：该基因具有泛酸激酶结构特征，氨基酸序列及核酸序列与同源物种相似度高，表明该基因在同源物种进化上高度保守，推测其在细胞质中起作用，可能与细胞代谢中的能量提供有关。

关键词： 白及；泛酸激酶基因；结构特征；功能预测

Abstract

Based on transcriptome sequencing data of Bletilla striata (B. striata), we used bioinformatics methods to analyze gene identification and sequence of PanK. First, we analyzed the isoelectric point, hydrophilicity and hydrophobicity, secondary and tertiary structure and domain of PANK protein in B. striata. Then we analyzed its subcellular localization, predicted phosphorylation site, signal peptide and transmembrane region. Finally, we built the evolutionary tree. The results showed that the PanK gene of B. striata contained an ORF of 2697 bp and encoded a total of 898 amino acids. The protein had a relative molecular mass of 99405.55 Da, which predicted to be a hydrophilic protein with an isoelectric point (pI) of 5.79. The protein had 11 threonine phosphorylation sites, and a conserved domain of pantothenate kinase (PLN02902 family protein), which contained 10 motifs. Then we found that this protein had no signal peptide and transmembrane region, and it was located in the cytoplasm through subcellular localization. The phylogenetic tree analysis showed that the PanK gene of B. striata was more closed to Dendrobium officinale (D. officinale). In conclusion, this research showed that thePanK gene had the structural characteristics of pantothenate kinase, and the sequence of amino and nucleic acid were highly similar to homologous species, which meant it was highly conservative in evolutionof homologous species. So we speculated that it played a role in the cytoplasm and might be related to the energy supply in cell metabolism.

Key words： Bletilla striata; PanK; Structure Characteristics; Functional Prediction

参考文献 References

[1] 陈磊, 唐成林, 程万,等.贵州产白及组培瓶苗分级标准及其验证_陈磊 [J]. 贵州农业科学, 2019, 47(10):110-4.

[2] 陈小璐,喻韬, 罗蓉. 泛酸激酶相关神经变性病的临床特征及分子生物学发病机制 [J]. 中华医学遗传学杂志, 2019, 36(2): 175-8.

[3] 万大群,赵仁全,刘海,等.白及的成分、药理作用和临床应用研究进展.中国药业,2017,26(2):93-96.

[4] Lim B C, Ki C S, Cho A, et al. Pantothenate kinase [J]. European Journal of Neurology, 2012, 19(4): 556-61.

[5] Awasthy D, Ambady A, Bhat J, et al. Essentiality and functional analysis of type I and type III pantothenate kinases of Mycobacterium tuberculosis [J]. Microbiology, 2010, 156(9): 2691-2710.

[6] Yuta O, Hiroki K, Munehiko A, et al. Role of prokaryotic type I and III pantothenate kinases in the coenzyme A biosynthetic pathway of Bacillus subtilis [J]. Canadian journal of microbiology, 2014, 60(5): 297-305.

[7] Kita A, Kishimoto A , Shimosaka T , et al. Crystal structure of pantoate kinase from Thermococcus kodakarensis [J]. Proteins. 2020, 88(5):718-724.

[8] 杨延辉,赵建新,王浩,等. 泛酸激酶及其抑制剂的研究进展 [J]. 药学进展, 2014, 38: 641-8.

[9] 沈丹. 泛酸激酶的克隆鉴定及其与泛酸类似物的作用 [J]. 清华大学学报, 2008.03, 48: 403-7.

[10] 张鹭,王庆忠,徐颖,等. 结核分枝杆菌泛酸激酶的克隆表达及酶学性质 [J]. 第四军医大学学报, 2006, 27(23): 2135-8.

[11] Arcus V L, Lott J S, Johnston J M, et al. The potential impact of structural genomics on tuberculosis drug discovery [J]. Drug Discovery Today, 2006, 11(1-2): 28-34.

[12] Ma L Y, Wang L, Yang Y M, et al. Novel gene mutations and clinical features in patients with pantothenate kinase‐associated neurodegeneration [J]. Clinical Genetics, 2015, 87(1): 93-5.

[13] Tanteles G A, Spanou-Aristidou E, Antoniou C, et al. Novel homozygous PANK2 mutation causing atypical pantothenate kinase-associated neurodegeneration (PKAN) in a Cypriot family [J]. Journal of the Neurological Sciences, 2014, 340(1-2): 233-6.

[14] Kimura Y, Kamimoto T, Tanaka N. Enzymatic Characteristics of a Polyphosphate/ATP-NAD Kinase, PanK, from Myxococcus xanthus [J]. Current Microbiology, 2019, 77(2): 173-178.

[15] Jeong S Y, Hogarth P, Placzek A, et al. 4’-Phosphopantetheine corrects CoA, iron, and dopamine metabolic defects in mammalian models of PKAN [J]. EMBO Molecular Medicine, 2019, 11: 1-17.

[16] Pan Y, Li L, Xiao S, etal. Callus growth kinetics and accumulation of secondary metabolites of Bletilla striata Rchb.f. using a callus suspension culture[J]. PLoS ONE, 2020, 15(2):e0220084.

[17] Li L, Liu H, Wen W, et al. Full Transcriptome Analysis of Callus Suspension Culture System of Bletilla striata [J]. Frontiers in Genetics, 2020, 11: 995.

[18] Liu H, Wu M, Zhu D, et al. Genome-Wide analysis of the AAAP gene family in moso bamboo (Phyllostachys edulis) [J]. Bmc Plant Biology, 2017, 17(1): 1-18.

[19] 郑培义. 酿酒酵母辅酶A生物合成蛋白Cab2（磷酸泛酰半胱氨酸合成酶）结构生物学研究[D].中国科学技术大学,2019.

[20] Temperature-Induced Extended Helix/Random Coil Transitions in a Group 1 Late Embryogenesis-Abundant Protein from Soybean [J]. Plant Physiology, 2002, 128(3): 822-832.

[21] Yao J, Subramanian C, Rock CO, etal. Human pantothenate kinase 4is a pseudo-pantothenate kinase [J]. Protein Sci. 2019;28(6):1031-1047.

引用本文

李清清, 黄恻隐, 范娠源, 刘海, 汪牧非, 徐德林, 白及泛酸激酶基因的生物信息学分析[J]. 细胞与分子生物学研究, 2021; 1: (1) : 26-32.

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